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ICAN analytics

 

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ican-analytics

This core facility is a molecular analytic platform dedicated to high throughput molecular phenotyping (latest “omics” technologies) and  includes “omics” Core Labs: Lipidomics, Metabolomics and Flow Cytometry.

There are ongoing efforts with ICAN INFO bioinformatics platform to integrate complementary gene array with the lipid and metabolomics data to decipher coordinately regulated genes and pathways and study of fluxes of lipids and metabolites through the  network.

 

1 – The Lipidomics Core lab  (Leaders: Dr Philippe Lesnik, Anatol Kontush (Dr Inserm) Scientific Director).

The lipidomics Core Lab is dedicated to analyze a broad range of lipid metabolites  in biological fluids, tissues and culture cells to determine their specific relevance to cardiometabolic diseases.

In order to screen a broad range of lipid metabolites, from highly hydrophobic to more water soluble molecules, the Core Lab utilizes two screening instruments able to quantify more than 150 molecular species of phospholipids and sphingolipids by LC-MS/MS, as well as fatty acids, plasmatic and fecal sterols by GC/MS.

In a near future, the Core Lab aims to develop a methodology for the determination of neutral lipid profile by LC/MS/MS (triacylglycerides, diacylglycerides and cholesteryl esters).

 

2 – The Metabolomics Core lab   (Leaders: Dr Philippe Lesnik, Dominique Gauguier , (Dr  Inserm) Scientific Director).

The metabolomics Core Lab is dedicated to analyze a broad range  of polar and non-polar metabolites in biological fluids, tissues and culture cells to determine their specific relevance to cardiometabolic diseases.

Following the recent award of an equipment grant by the region Ile de France to Dominique Gauguier, ICAN has acquired a high resolution mass spectrometer (UPLC/Q-Exactive) dedicated to metabolomics studies. The Core Lab  plans to analyze polar metabolites by targeted approaches (alignment with data derived by 1H NMR profiling and quantification of amino acids, acylcarnitines and hexoses) as well as by non-targeted approaches (collaborations with CEA, Saclay and Imperial College London).

 

3 – The Flow Cytometry Core Lab  (Leaders: Dr Philippe Lesnik, Sébastien André (MCU-UPMC) Scientific Director)

The flow cytometry Core Lab is dedicated to provide experimental design, flow cytometric analysis and cell sorting service through its flow cytometry instrumentation and expertise.

The flow cytometers in place allow simultaneous multiparametric analysis of many thousands of cells per second, phenotypic analysis  (cell-surface/intracellular protein expression, phosphorylation) and functional studies e.g., cell cycle, apoptosis, cytokine production and cell proliferation analysis on a variety of cell types, such as whole blood, tissue cells and cell lines. The Core Lab has developed standard set of surface marker panels for the immunomonitoring of blood and metabolic tissue-resident immune cells for clinical studies. In addition, it has  set up tools dedicated to cells enriched in lipids, which requires specific approaches both in the analysis (treatment of auto-fluorescence) as well as in the cleaning and maintenance of  cytometers (tissue leukocytes especially macrophages are extremely sticky).

The Core Lab is also equipped with single-cell suspensions preparation devices  for dissociation of tissues (liver, gut, spleen, adipose tissue, heart) into sterile single cell suspensions and for cells immunoselection.